International Council for Education, Research and Training

Brewing Health Benefits: A Comparative Study Of Black, Herbal, And Green Tea On Rat Physiology

Talabi, Olaoluwa Temitope1, Emmanuel, Ademilua T.2, Oluwakemi, Akande Toluwalope3, Oluwole, Ruth A4, Osilesi, Odutola 5, Adebawo, Olugbenga O6, and Talabi, Joseph Moyinoluwa7

1Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria.

2,3,4,5&6Department of Biochemistry, Benjamin Carson Snr. College of Medicine, Babcock University.

7Department of Religions and Peace Studies, Lagos State University, Ojo


This study compared the physiological effects of black, herbal, and green tea in rats to determine prospective health advantages. Animals were divided into eight groups of six male rats each. In the morning and evening, the negative control group was intubated with 1ml of distilled water, with rat chow and water. The remaining six groups got each type of tea (black, herbal, and green) in the morning and evening for 14 days while physiological data were recorded. Green tea showed the most significant lipid profile and liver function improvement among the three tea varieties. The findings imply that all three tea varieties may have health advantages, particularly in enhancing liver health and lipid profiles. The highest impacts on lipid profile and body weight reduction were specifically shown with green tea.

Keywords: Male rats, Liver function, lipid profile, Black tea, Green Tea, Herbal Tea.

Impact statement

This study investigated the physiological effects of black, herbal, and green tea consumption in rats, revealing significant insights into potential health benefits. The research highlighted that all three tea varieties, particularly green tea, can positively impact lipid profiles and liver function. Notably, green tea exhibited the most significant improvement in lipid profiles and liver health among the tested teas. The findings underscore the potential of these teas in enhancing liver health and promoting weight reduction. This comparative analysis deepens our understanding of tea’s health benefits beyond the widely studied green tea, holding implications for managing conditions such as cardiovascular disease and non-alcoholic fatty liver disease, with broad public health significance.

About the Author

Olaoluwa T. Talabi, Ph.D, Corresponding Author: 

Olaoluwa Temitope Talabi is a biochemistry enthusiast with a strong academic background and extensive experience in prestigious institutions. She holds a Doctorate Degree in Nutritional Biochemistry and currently works as a Senior Lecturer in the Biochemistry Department at the College of Medicine, University of Lagos. In addition to her role as a lecturer, Olaoluwa is actively engaged in research as a Research associate at the Centre for Genomics of Non-communicable Diseases and Personalized Healthcare (CGNPH). 

In addition to her academic and research roles, Olaoluwa actively participates in various professional societies, including the Nigerian Society of Biochemistry and Molecular Biology (NSBMB), The South Africa Society for Biochemistry and Molecular Biology (SASBMB), and the American Society of Nutrition (ASN), among others. She is also dedicated to pursuing excellence.

Olaoluwa’s passion lies in pushing the boundaries of scientific knowledge, particularly in the domains of nutrition, genomics, non-communicable diseases, and personalized healthcare. She frequently engages in conferences and workshops to enrich his knowledge in research methodologies, educational practices, and ethical publication standards. Olaoluwa aims to disseminate knowledge through his research papers and contribute to the scientific community.

With her wealth of academic expertise, publication track record, and commitment to the scientific community, Olaoluwa is well-prepared to continue making an impact in the fields of biochemistry and genomics. 


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Web of Science Researcher ID: HMD-4824-2023.

Joseph Moyinoluwa Talabi, Ph.D,

Joseph Moyinoluwa TALABI holds a PhD in Philosophy of Religion from Lagos State University. He is a Lecturer in the Department of Religions and Peace Studies, Faculty of Arts, Lagos State University, Ojo, Lagos, where he lectures and researches the social value of religions. His areas of interest include but are not limited to, Philosophy of Religion, Psychology, Politics, Theology, Health and Sociology of Religion (Religion and Social Institutions). He has convened a great deal of experience in his few years in academic learning, publishing in local and high-impact factor journals indexed in Scopus and Web of Science. Some of these articles are in Q1 and Q2 Journals. Such as; Taylor and Francis, Human Vaccine & Immunotherapeutics, Oxford University, Telematics and Informatics, Asian and African Studies, and Health Promotion International. Is a member of the Editorial Team; Ianna Journal of Interdisciplinary Studies. He also reviews the Journal of Religion & Spirituality in Social Work, Journal of Religion & Health (JORH), and World Journal of Education and Humanities.

He is also a Priest in the Anglican Communion, Church of Nigeria, Diocese of Lagos West, with experience of ten years in an Ordained ministry.

ORCID ID: 0000-0002-3321-0835

Scopus Author ID: 57222136064

Researcher ID: AAX-2918-2021

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Ademilua T. Emmanuel

Ademilua T. Emmanuel, is a dedicated alumnus of Babcock University, with a passion for academic excellence and a thirst for knowledge. His educational journey has equipped him with a solid foundation and a diverse range of skills that he is eager to apply to real-world challenges. His commitment to lifelong learning and my alma mater’s values continue to drive his  academic pursuits.

Akande Toluwalope O

Akande Toluwalope O, is a dedicated and enthusiastic individual who completed her studies at Babcock University, specializing in the field of Biochemistry. Throughout her academic journey, she displayed a strong commitment to learning and personal growth, consistently striving for excellence in both her studies and extracurricular activities.

As a proud alumnus of the Biochemistry Department at Babcock University, she is equipped with a solid foundation in biochemistry principles and practical skills. Her time at the university has nurtured her curiosity and honed her critical thinking abilities, preparing me to contribute meaningfully to the scientific community and the world at large.

Oluwole Ruth A

Oluwole Ruth A, is an enthusiastic and dedicated individual who has completed her academic journey at Babcock University, specializing in the field of Biochemistry. Throughout my time at the university, I have consistently demonstrated a strong passion for learning, a commitment to personal growth, and an unwavering dedication to excellence.

As an alumnus of the esteemed Biochemistry Department at Babcock University, she is well-equipped with a comprehensive understanding of biochemistry principles and practical skills. My educational experiences have fostered my analytical thinking and problem-solving abilities, positioning me to make valuable contributions to the scientific community and beyond.

O.O Adebawo: Professor of Toxicological Biochemistry, Olabisi Onabanjo University (OOU) and Current Dean, Benjamin Carson (Snr) School of Medicine, Babcock University, Ilishan Remo

Professor O.O Adebawo, is an esteemed academic specializing in the dynamic field of Toxicological Biochemistry. He holds the position of Professor within the distinguished Olabisi Onabanjo University (OOU), where he is deeply engaged in advancing knowledge and fostering excellence.

As a Professor of Toxicological Biochemistry, he is driven by a profound commitment to both education and research. At Olabisi Onabanjo University, he passionately shares his expertise with students, equipping them with a comprehensive understanding of the intricate connections between biochemistry and toxicology.

In addition to his academic role at OOU, he is honoured to serve as the Dean of the Benjamin Carson (Snr) School of Medicine at Babcock University, located in the vibrant community of Ilishan Remo. In this esteemed capacity, he oversees the academic and administrative aspects of the School of Medicine, striving to cultivate a nurturing environment for learning and research.

His research pursuits encompass a wide spectrum of toxicological biochemistry, as he seeks to unravel the complex mechanisms underlying chemical exposure and its repercussions on living systems. By contributing valuable insights to this field, he aims to fortify our ability to safeguard public health and the environment.

The esteemed institutions of Olabisi Onabanjo University and Babcock University provide an enriching backdrop for his academic endeavours. Within these institutions, he collaborates with fellow scholars and researchers, fostering an environment of innovation, inquiry, and discovery.

Late O. Osilesi: Professor of Nutritional Biochemistry, Biochemistry Department, Babcock University, Ilishan Remo, and Former Vice Chancellor, Olabisi Onabanjo University, Ago Iwoye (OOU)

In Memoriam: The academic community mourns the loss of Late O. Osilesi, a distinguished and accomplished figure in the realm of Nutritional Biochemistry. With a rich academic and administrative background, Late Professor Osilesi’s legacy resonates through his contributions to both Babcock University and Olabisi Onabanjo University (OOU). 

As a Professor of Nutritional Biochemistry at Babcock University, Ilishan Remo, Late Professor Osilesi left an indelible mark on the institution. His expertise, dedication, and passion for the field were evident in his teaching, research, and mentorship. His influence extended beyond the classroom, inspiring countless students to delve deeper into the intricate world of nutritional sciences.

Late Professor Osilesi’s tenure as the Vice Chancellor of Olabisi Onabanjo University, Ago Iwoye, showcased his visionary leadership and commitment to higher education. Under his guidance, the university experienced growth and transformation, positioning OOU as a hub of learning and innovation.

As we reflect on Late Professor Osilesi’s impactful journey, we remember his scholarly pursuits, his dedication to advancing nutritional biochemistry, and his role in shaping the educational landscape. His legacy will continue to inspire generations of academics and learners, leaving an enduring imprint on the academic community.

Though he is no longer with us, Late Professor Osilesi’s contributions remain alive in the halls of academia and in the hearts of those he touched. Our thoughts and condolences are with his family, friends, colleagues, and students during this time of remembrance.

  • Babu, P. V., & Liu, D. (2008). Green tea catechins and cardiovascular health: An update. Current medicinal chemistry, 15(18), 1840-1850.
  • Bahram Pourghassem Gargari, B., Dehghan, P., Aliasgharzadeh, A., & Asghari Jafar-Abadi, M. (2015). Effects of black tea consumption on blood cholesterol concentrations in rats. Journal of medicinal food, 18(3), 278-284.
  • Chacko, S. M., Thambi, P. T., Kuttan, R., & Nishigaki, I. (2010). Beneficial effects of green tea: A literature review. Chinese Medicine, 5(1), 13.
  • Chen, H. C., Hsu, Y. J., Liou, S. S., Chen, C. Y., Tsai, Y. C., & Huang, C. C. (2015). Effect of oolong tea on enhancing cardioprotective and antioxidant capability in hyperlipidemic rats. Journal of agricultural and food chemistry, 63(20), 4882– 4889.
  • Chen, M. F., Huang, C. C., Liu, J. R., & Yang, H. T. (2018). Effects of green tea polyphenols supplementation on liver damage induced by high-intensity exercise in rats. Archives of physiology and biochemistry, 124(2), 154-159.
  • Chen, Z. Y., Zhu, Q. Y., Tsang, D., Huang, Y., & Ho, K. Y. (2018). Flavonoid content in tea and its association with its antioxidant capacity. Journal of Agricultural and Food Chemistry, 46(2), 156-162.
  • Davies, M. J., Judd, J. T., Baer, D. J., Clevidence, B. A., Paul, D. R., & Edwards, A. J. (2018). Black tea consumption reduces total and LDL cholesterol in mildly hypercholesterolemic adults. The Journal of nutrition, 138(10), 890–897.
  • Fetrow, C. W., & Avila, J. R. (2000). The Complete Guide to Herbal Medicines. Pocket Books.
  • Grove, K. A., Lambert, J. D., & Qi, Y. (2018). Polyphenols found in tea and their health benefits. In Tea in Health and Disease Prevention (pp. 39–55). Academic Press.
  • Henning, S. M., Fajardo-Lira, C., Lee, H. W., Youssefian, A. A., Go, V. L., & Heber, D. (2012). The Catechin content of 18 teas and a green tea extract supplement correlates with the antioxidant capacity. Nutrition and Cancer, 43(2), 133-144.
  • Hursel, R., & Westerterp-Plantenga, M. S. (2010). Catechin- and caffeine-rich teas for control of body weight in humans. The American Journal of clinical nutrition, 91(3), 557-563.
  • Hursel, R., Viechtbauer, W., & Westerterp-Plantenga, M. S. (2011). The effects of green tea on weight lo weight maintenance: a meta-analysis. International Journal of obesity, 33(9), 956-961.
  • Janssens, P. L., Hursel, R., Martens, E. A., & Westerterp-Plantenga, M. S. (2016). Acute effects of capsaicin on energy expenditure and fat oxidation in negative energy balance. PloS one, 11(3), e0150757.
  • Joubert, E., de Beer, D., & Manley, M. (2008). Antioxidant and antimutagenic properties of aqueous extracts from Rooibos tea (Aspalathus linearis). Journal of Agricultural and Food Chemistry, 56(17), 8115-8120.
  • Jówko, E., Długołęcka, B., Makaruk, B., & Cieśliński, I. (2019). Green tea catechins supplementation decreases oxidative stress markers in healthy men. Journal of Physiology and Pharmacology, 70(3), 421-427.
  • Kim, K. H., Tsao, R., & Yang, R. (Flavour Flavor quality of green tea and green tea products. In Green tea (pp. 127–144). Springer, Cham.
  • Li, F., Yao, M., Li, J., Sun, Z., Han, Y., Liu, J., & Chen, Y. (2019). Black tea: chemical analysis and stability. Food Research International, 116, 674-682.
  • Li, S., Han, Y., Yu, Q., Lv, X., Yang, B., Zhao, W., … & Wang, H. (2015). Green tea extract improves insulin resistance and hepatic lipid accumulation in obese mice by regulating IRS/JNK/FOXO1 pathway and lipogenic gene expression. European Journal of nutrition, 54(–), 827–837.
  • Liu, Y., Zeng, G., Zhang, L., Lu, X., Zhou, Z., & Liang, J. (2021). Health Benefits of Tea: A Comprehensive Review. Journal of Food Science, 86(–), pp. 797– 812.
  • Mansour, M. S., Ni, Y. M., Roberts, A. L., Kelleman, M., Roychoudhury, A., & St-Onge, M. P. (2019). Tea consumption is associated with a reduced risk of early death and cardiovascular disease in the US population. The Journal of nutrition, 149(10), 1872-1881.
  • McKay, D. L., & Blumberg, J. B. (2006). A review of peppermint tea’s bioactivity and potential health benefits (Mentha piperita L.). Phytotherapy Research, 20(8), 619–633.
  • Tsai, C. F., Kuo, W. W., Ho, Y. J., & Huang, C. Y. (2014). Black tea extract improves high-fat diet-induced obesity in rats by alleviating lipogenesis by regulating the AMPK-PACC1-PGC1α pathway. Journal of agricultural and food chemistry, 62(21), 4793–4801.
  • Wang, L., Meng, X., Zhang, F., & Zhu, R. (2019). Health benefits of dietary polyphenols and mechanisms of action: a systematic levitical reviews in food science and nutrition, 59(16), 2680-2701.
  • Wes Westerterp-Plantenga MS, Nieuwenhuizen A, Tomé D, Soenen S, Westerterp KR. Dietary protein, weight loss, and weight maintenance. Annu Rev Nutr. 2009; 29:21-41. doi: 10.1146/annurev-nutr-080508-141056. PMID: 19400750.
  • Zhang, X., Qi, Y., Guo, X., Su, Z., Xia, T., Tong, L., & Zhang, Z. (2017). The effect of green tea extract supplementation on liver enzymes in rats fed a high-fat diet. Journal of food science and technology, 54(6), 1422–1429.
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