International Council for Education, Research and Training

Phytochemical Screening and Anti – oxidant Activities of the Roots and Leaves of Citrus Aurantifolia (Lime orange) grown in Imane, Olamaboro, Kogi, Nigeria

Omale, Moses Ojochogwu1

Isah, Aminu Agih2

Abuh, Leonard Omachoko3

Omale, Ugbedeojo Emmanuel4

1Department of Biology, Kogi State College of Education, Ankpa.

2Department of Chemistry, Kogi State College of Education, Ankpa.

3&4Department of Integrated Science, Kogi State College of Education, Ankpa



Traditional doctors have been utilizing the advantages of the potency of natural plants in the treatment and prevention of several ailments for decades without them or their patience knowing the active ingredients responsible for the potency. Hence the need to evaluate and understand the medicinal bio – active substances present in plants. This work analyses the phytochemical substances and the anti – oxidant activities of the roots and leaves of citrus aurantifolia. The plant parts were collected, dried, pulverized and extracted in absolute ethanol. The extracts collected were analyzed for bio – active substances and antioxidant activities. The results show the presence of alkaloids, carbohydrates, glycosides, saponins, phenols, tannins, flavonoids, protein and amino acid in both the roots and leaves of citrus aurantifolia. The KMnO4 scavenging effects of the plant parts show them to displayed effective potentials as anti – oxidants as the % KMnO4 radical scavenging competes favorably; (root – 85.60% and leaves – 89.88%) with the reference ascorbic acid (83.82%). This indicate that the plant parts can scavenge free radical from the body. These finding denotes that the root and leave of citrus aurantifolia can be a good source of therapeutic for health management. 

Keywords: Phytochemicals, Antioxidant, lime orange, Citrus Aurantifolia.


Impact Statement

Elucidation of the bioactive substances and biological activities of plants around us is of great importance. The leaves and roots of Citrus aurantifolia have been a source of therapeutic in health management for decades in our locality with the traditional doctors and their patients having little or no knowledge of the active ingredients necessitating the medicinal potency. This work has justified that the plant parts are endowed with bioactive substances with strong antioxidant potentials. The scavenging abilities and the presence of medical components in indicate the potency of the leaves and root of Citrus aurantifolia in the treatment and prevention of several ailments in our locality for health management. This work has added to the medicinal value of the plant parts for the wellbeing of humanity in and around our community.


About Author/s

Omale, Moses Ojochogwu was born on 27/02/1981. I attended LGEA primary school Enjema, Ankpa Local Government, Kogi State. I went to Federal Government College, Ugwolawo and on completion in 1995 I proceeded to Kogi State of College of Education where I obtained Nigeria Certificate in Education (NCE) in Biology/Chemistry in 1999. My first degree was obtained from the University of Nigeria, Nsukka, Enugu State with a Bachelor of Science degree (BSc) in Biology Education in 2005. My second degree was in Science Education from Benue State University, Makurdi where I graduated in 2012 with a Master’s degree in Science Education (MEd Sc.)  Currently I am a PhD candidate in Benue State University Makurdi, Nigeria, a professional teacher, registered with Teacher’s Registration Council of Nigeria (TRCN). I am a well-groomed educator and lectures currently in the Department of Biology, Kogi State College of Education, Ankpa, Nigeria.

Orcid ID:0009-0002-3200-1510

Abuh Leonard Omachoko was born on the 27th Sept. 1972. He attended several primary school educations but completed at LGEA primary school in Oguma, Bassa, Kogi State. He went to St Charles College, Ankpa and on completion he proceeded to Kogi State of College of Education where he obtained Nigeria Certificate in Education in Biology / Chemistry in 1994. In 1995 he proceeded to Abubakar Tafawa Balewa University, Bauchi where he obtained a Bachelor of technology degree (B. Tech) in Industrial Chemistry in 1999. He furthered his education in Kogi State University, Anyigba where he graduated in 2017 with a Master’s degree (M. Sc) in Industrial Chemistry. Currently he is studying Industrial Chemistry at PhD in the Federal University of Lafia, Nassarawa, Nigeria. He is a seasoned educator and lectures currently in the Department of Integrated Science, Kogi State College of Education, Ankpa, Nigeria.

Orcid ID: 0000 – 0003 – 3017 – 1733

Omale Emmanuel was born on the 21st April, 2007 in Imane, Olamaboro L. G. A. of Kogi State, Nigeria. I attended Prison Model Primary and Secondary School between 2007 and 2011, and 2012 and 2018 respectively. I went to Kogi State College of Education, Ankpa and obtained Nigeria Certificate of Education (NCE) in Integrated Science/Chemistry in 2021 where I gained my research experience as a supervisee.

Orcid ID: 0009-0002-7454-7722

Isah Aminu Agih is my name born on 11/11/1973. I attended Iyaji Commercial Secondary School Abejukolo in Omala Local Government Area, kogi state in 1988. I graduated from the Federal polytechnic, Idah, in 1997. I am currently a Technologist in Chemistry Department, kogi state college of education, Ankpa. I am an analyst and a researcher.

Orcid ID: 0000-0002-9675-3247



  1. Namani, A., Baqir, J., E., Abri, A., Al Hubaishi, A., T., . . . Khan, S. A. (2018). Phytochemical screening phenolic content and antioxidant activity of citrus aurantifolia L. leaves grown in two regions of Omas. Pharmaceutical Sciences, 14, 47–58.

  2. Bukola, C. A., Temitayo, O. A., & Oubusola, A. A. (2016). Phytochemical composition and comparative evaluation of antimicrobial activities of citrus ayrantifolia and its silver Nano particls. Nigeria Journal of Pharmaceutical Research, 12(1).

  3. Oikeh, E. I., Omoregie, E. S., Oviasogie, F. E., & Oriakhi, K. (2016). Phytochemical, antimicrobial, and antioxidant activities of different citrus juice concentrates. Food Science and Nutrition, 4(1), 103–109. doi:10.1002/fsn3.268.

  4. Isaac, K. A., Emmanuel, O., Abraham, Y. M., Francis, M. S., Francis, A., & Linda, M. S. (2016). Development and validation of radical scavenging antioxidant assay using potassium permanganate. Journal of Scientific and Innovative Research, 5(2), 36–42.

  5. Jokha, A., Esra, B., Ajwaa, A., Tamadher, A., Asif, H., & Shah, A. K. (2018). Phytochemical screening, phenolic content and antioxidant activity of citrus aurantifolia l. leave grown in two regions of omans. Iranian Journal of Phymaceutical Sciences, 14(1), 27–34.

  6. Shaikh, J. R., & Patil, M. K. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603–608. doi:10.22271/chemi.2020.v8.i2i.8834

  7. Kazeem, M., Bukola, I., H. A., Oladokun, T. I., Bello, A. O., & Malik, M. A. (2020). citrus aurantifolia (chrism) swingle fruit extract inhibit the activities of polyol pathway enzyme. E Food, 1, 310–315. Doi.10.29911/efood.k.200824.001

  8. Leonard, O. A., Salifu, V., & Samuel, A. S. (2022). Dyestuff utilization of the bark of Parkia biblobosa grown in Ankpa, Koogi, Nigeria. FUW trend in science and technology journal, 7(2), 862–866.

  9. Maha, S. A., Nour, M. A., Sausan, S. A., Tanveer, A., & Shah, A. K. (2018). Chemical composition and in – Vitro antioxidant and antimicrobial a ctivities of essential of citrus aurantifolia leaves grown in Oman. Journal of Taiban, 13(2), 108–112.

  10. Micheal, C. (2016). Citrus aurantifolia (P. rosea). Retrieved from https://www.uses.plantnetproject.orgcitrus_au…. Oncology and Therapy 15/09/2023.

  11. Mohammad, K. N., Mahmood, N. D., Omoye, B., Abdurrahmam, A., Kibiru, S., Ibrahim, B., & Umar, K. A. (2018). Phytochemical screening and antioxidant activities of citrus sinensis (L.) osbeck (orange) and Citrus aurantifolia (cistm) swingle (lime) stem from bacterial associated with Dental cares. Advances in Microbiology, 8(4), 1–4.

  12. Lawrence, A. A. (2023). Transition from fossil fuel to clean energy: A must achievable project in Sub-Saharan Africa. Shodh Sari-An International Multidisciplinary Journal, 41–49. doi:10.59231/SARI7573

  13. Asowata-Ayodele, M. A., Dabesor, P. A., & Afolabi, B. Phytochemical Compositions and Antimicrobial Activities of Citrus sinensis and Citrus aurantifolia Peels on Selected Pathogenic Bacteria Isolated from Jollof Rice. International Journal of Pathogen Research, 1–7. doi:10.9734/ijpr/2019/v2i330075

  14. Nazer, H. O. E., Ragaa, S. M. A., & Saad, M. H. A. (2018). Phytochemical screening and assessment of antioxidant and antimicrobial activities of the root extract of tree Sudanese citrus species. Americal Journal of Research Communication, 6(4), 1–9.

  15. Prashant, T., Bimlesh, K., Mandeep, K., Gupreet, K., & Harleen, K. (2011). Phytochemical screening and extraction. A review. International Pharmaceutical Sciencia, 1(1), 98–106.

  16. Swanding. (2021). Standardization of 70% ethanol extract and 96% lime leaves as antioxidant with DPPH and FRAP. J. Pharmacogen. Phytochem. G/F. Nafisa, S, and Gangga, E, 10, 47–52.

  17. Rahmiati, N., Sari, R., & Wahyuni, T. S. The Phytochemical and Antioxidant Activity Evaluation of Lime (Citrus aurantifolia) Juice Powder. Journal Farmasi Galenika. doi:10.22487/j24428744..v.i.16347


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